Effect of Yang He Decoction on Treatment of Bone Tuberculosis via Phosphoinositide 3-Kinases/Protein Kinase B and Mitogen-Activated Protein Kinase Signaling Pathways

Yang He Decoction (YHD), a classical Chinese medicine prescription, is used to treat bone and joint diseases. However, there are few mechanism studies for YHD on the use of tuberculosis (BT) corresponding action YHD. In present study, chemical ingredients targets were revealed by network pharmacology method, an ingredient–target–disease was visualized analyzed. Then, gene ontology enrichment analysis Kyoto Encyclopedia Genes Genomes pathway carried out. addition, molecular docking performed clarify binding key active targets. Further, in vitro model Mycobacterium tuberculosis-induced BT established, inhibitory effect differentiation RAW 264.7 cells into osteoclasts investigated. A total 138 50 between identified. The phosphatidylinositol 3-kinase–protein kinase B (PI3K–Akt) mitogen-activated protein (MAPK) signaling pathways involved anti-BT Moreover, results showed that inhibited osteoclasts. decreased levels tumor necrosis factor-α interleukin-1β, increased superoxide dismutase glutathione peroxide, level malondialdehyde. messenger RNA expression PI3K/Akt, p38 MAPK, c-Jun N-terminal kinase. These findings show promising agent suppresses PI3K/Akt MAPK inhibit ameliorate inflammation oxidative stress.